Yeast Saccharomyces cerevisiae as a tool in cloning and analysis of fungal genes applications for biomass hydrolysis and utilisation by Anu Saloheimo

Cover of: Yeast Saccharomyces cerevisiae as a tool in cloning and analysis of fungal genes | Anu Saloheimo

Published by VTT Technical Research Centre of Finland in Espoo [Finland] .

Written in English

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  • Saccharomyces cerevisiae -- Genetic engineering.,
  • Trichoderma reesei -- Genetic engineering.,
  • Cellulase.,
  • Molecular cloning.

Edition Notes

Book details

StatementAnu Saloheimo.
SeriesVTT publications -- 541., VTT julkaisuja -- 541.
ContributionsValtion teknillinen tutkimuskeskus.
The Physical Object
Pagination1 v. (various pagings) :
ID Numbers
Open LibraryOL18219266M
ISBN 109513864006

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Yeast Saccharomyces cerevisiae as a tool in cloning and analysis of fungal genes: Applications for biomass hydrolysis and utilisation Article (PDF Available) in VTT Publications January Yeast Saccharomyces cerevisiae as a tool in cloning and analysis of fungal genes: Applications for biomass hydrolysis and utilisation.

By Anu Saloheimo. Get PDF (2 MB) Publisher: Helsingfors universitet. Year: OAI identifier: oai: Primers‐4‐Yeast creates, for the first time, a consistent database of primers for yeast transformation and its verification, thus relieving researchers from unnecessary manual primer design.

Our web tool facilitates fast and accurate primer planning for large sets of target genes and targeting :// About SGD. The Saccharomyces Genome Database (SGD) provides comprehensive integrated biological information for the budding yeast Saccharomyces cerevisiae along with search and analysis tools to explore these data, enabling the discovery of functional relationships between sequence and gene products in fungi and higher :// Saccharomyces cerevisiae has been developed as a model eukaryotic organism for a number of reasons, for example.

Saccharomyces cerevisiae is a small single cell with a doubling time of 30 °C of –2 h and importantly can be cultured easily. Consequently, they permit the rapid production and maintenance of multiple strains at low ://   The budding yeast Saccharomyces cerevisiae is one of the most intensively studied eukaryotic model organisms.

It is a single cell organism with a The Saccharomyces sensu stricto species complex (Vaughan‐Martini & Martini, ) contains some of the most important species for the food industry, namely, S. cerevisiae (Meyen ex EC Hansen), the agent of wine, bread, ale and weiss beer, and sake fermentations, Saccharomyces bayanus (wine and cider fermentations) and Saccharomyces   Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of its long history in industrial fermentations.

However, until recently, most synthetic biology systems have focused on bacteria. While there is a wealth of resources and literature about the biology of yeast, it can be daunting to navigate and extract the tools needed for engineering applications.

Here   The genes encoding the seven GFPs were optimized for expression in the yeast S. cerevisiae (yEGFP, yAcGFP1, yTagGFP2, ymUkG1, yZsGreen, ymWasabi and ymNeonGreen) (Supplementary Table S1) and Saccharomyces cerevisiae is the most thoroughly investigated eukaryotic microorganism, which aids our understanding of the biology of the eukaryotic cell and hence, ultimately, human biology.

For several centuries, S. cerevisiae has been used in the production of food and alcoholic beverages, and today this organism is also used in a number of different processes within the pharmaceutical Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cerevisiae.

Directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). Together with a fold increase in k cat, the total activity was enhanced fold.

Specific activities of MtL mutants toward 2,2′-azino-bis(3-ethylbenzthiazoline-6   The term ‘sake yeast’ is generally used to indicate the Saccharomyces cerevisiae strains that possess characteristics distinct from others including the laboratory strain SC and are well suited for sake brewery.

Here, we report the draft whole-genome shotgun sequence of a commonly used diploid sake yeast strain, Kyokai no. 7 (K7). Yeast Saccharomyces cerevisiae as a tool in cloning and analysis of fungal genes: Applications for biomass hydrolysis and utilisation Article Full-text available Abstract.

The budding yeast Saccharomyces cerevisiae has many advantages as a model system, but until recently high-resolution microscopy was not often attempted in this organism. Its small size, rounded shape, and rigid cell wall were obstacles to exploring the cell biology of this model ://   Background Model organisms are used for research because they provide a framework on which to develop and optimize methods that facilitate and standardize analysis.

Such organisms should be representative of the living beings for which they are to serve as proxy. However, in practice, a model organism is often selected ad hoc, and without considering its representativeness, because a ?id=/ While the pace of discovery of human genetic variants in tumors, patients, and diverse populations has rapidly accelerated, deciphering their functional consequence has become rate-limiting.

Using cross-species complementation, model organisms like the budding yeast, Saccharomyces cerevisiae, can be utilized to fill this gap and serve as a platform for testing human genetic variants.

To this Ancient fermented food has been studied based on recipes, residue analysis, and ancient-DNA techniques and reconstructed using modern domesticated yeast. Here, we present a novel approach based on our hypothesis that enriched yeast populations in fermented beverages could have become the dominant species in storage vessels and their descendants could be isolated and studied :// SUMMARY Comprehensive knowledge regarding Saccharomyces cerevisiae has accumulated over time, and today S.

cerevisiae serves as a widley used biotechnological production organism as well as a eukaryotic model system. The high transformation efficiency, in addition to the availability of the complete yeast genome sequence, has facilitated genetic manipulation of this microorganism, and new In the past several decades the budding yeast Saccharomyces cerevisiaehas emerged as a prominent model for aging research.

The creation of a single-gene deletion collection covering the majority of Yeast culture for maintaining and propagating various strains, such as Saccharomyces cerevisiae (S. cerevisiae) and Pichia pastoris (P. pastoris), requires specific formulations of complex media for use in cloning and protein offer a selection of yeast growth media for your specific application in both powder and ready-to-use liquid formats for convenience and ease of :// /microbiological-culture/ SUMMARY The traditional use of the yeast Saccharomyces cerevisiae in alcoholic fermentation has, over time, resulted in substantial accumulated knowledge concerning genetics, physiology, and biochemistry as well as genetic engineering and fermentation technologies.

cerevisiae has become a platform organism for developing metabolic engineering strategies, methods, and :// The budding yeast Saccharomyces cerevisiae is a powerful model organism for studying fundamental aspects of eukaryotic cell biology.

This Primer article presents a brief historical perspective on the emergence of this organism as a premier experimental system over the course of the past century. An overview of the central features of the S. cerevisiae genome, including the nature of its The yeast two-hybrid system is a powerful molecular genetic tool conceived by Fields and Song.

The article is a comprehensive set of methods designed to take the reader through a yeast two-hybrid analysis of your favorite gene (YFG). This article details the preparation for a screen, the screen itse   A sterol C reductase (erg) mutant of Saccharomyces cerevisiae was selected in a fen1, fen2, suppressor background on the basis of nystatin resistance and ignosterol (ergosta-8,dienol) erg allele segregated genetically as a single, recessive gene.

The wild-type ERG24 gene was cloned by complementation onto a kb fragment from a yeast genomic library, Aminopeptidase that preferentially cleaves di- and tripeptides.

Also has low epoxide hydrolase activity (in vitro). Can hydrolyze the epoxide leukotriene LTA(4) but it forms preferentially 5,6-dihydroxy-7,9,11,eicosatetraenoic acid rather than the cytokine leukotriene B(4) as the product compared to the homologous mammalian enzyme (in vitro).

«hide 10 20 30 40 50 msykvnssyp dsippteqpy masqykqdlq sniamatnse qqrqqqqqqq 60 70 80 90 qqqqqwinqp taensdlkek mnckntlney ifdfltkssl kntaaafaqd ahldrdkgqn pvdgpksken ngnqntfskv vdtpqgflye wwqifwdifn tsssrggsef aqqyyqlvlq eqrqeqiyrs lavhaarlqh daerrgeysn edidpmhlaa mmlgnpmapa vqmrnvnmnp ipipmvgnpi vnnfsippyn RNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae.

Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small Strains. Saccharomyces cerevisiae FLa was the wild-type strain used for all yeast-related manipulations.

The S. cerevisiae ura6 strain was derived from FLa and displayed a conditional thermosensitive and 5-fluorouracil-resistant phenotype (Liljelund and Lacroute, ).The strain used in these studies was also auxotrophic for His and Trp. Genotypes of the yeast strains were determined Gene Manipulations in Fungi combines a review of classical fungal genetics, contemporary research, and responsible speculation about the future.

This book focuses on yeasts and molds; because yeast is the primary model system for eukaryotes and that there is an elegant research on :// The strong overexpression or complete deletion of a gene gives only limited information about its control over a certain phenotype or pathway.

Gene function studies based on these methods are therefore incomplete. To effect facile manipulation of gene expression across a full continuum of possible expression levels, we recently created a library of mutant ://   Université de Fribourg - Home | | Université de Fribourg   FIGURE 1.

Cloning by homologous recombination. (A) In vitro homologous recombination of a linearized yeast plasmid and an insert through the creation of complementary single-stranded DNA overhangs by means of an exonuclease.

(B) In vivo recombination of a linearized yeast plasmid and an insert in budding yeast using homologous double-strandedDNA sequences introduced by :// Saccharomyces boulardii is a probiotic yeast often used for the treatment of GI tract disorders such as diarrhea symptoms. It is genetically close to the model yeast Saccharomyces cerevisiae and its classification as a distinct species or a S.

cerevisiae variant has long been discussed. Here, we review the main genetic divergencies between S. boulardii and S. cerevisiae as a strategy to Sodium and potassium are two alkali cations abundant in the biosphere. Potassium is essential for plants and its concentration must be maintained at approximately mM in the plant cell cytoplasm including under circumstances where its concentration is much lower in soil.

On the other hand, sodium must be extruded from the plant or accumulated either in the vacuole or in specific plant Previous studies have demonstrated that CRISPR-Cas9 technology can be used for efficient, marker-free genome editing in Saccharomyces cerevisiae.

However, introducing the 20mer guide sequence into yeast sgRNA expression vectors often requires cloning procedures that are   Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as well as light chain display plasmids in different haploid yeast strains followed by yeast mating.

Within this study, we aimed at implementing a focused The completion of the Saccharomyces cerevisiae genome project and molecular analysis of other fungal species has resulted in the identification of a growing number of yeast AP-1 transcription factors [].Characterization of these factors indicates that, like their mammalian counterparts, they activate gene expression in response to a variety of extracellular stimuli [1–4].

Cloning of the late genes in the ergosterol biosynthetic pathway ofSaccharomyces cerevisiae—A review Lipids, Vol. 30, No. 3 Effects of elevated expression of the CYP51 (PDM) gene on the sterol contents of Saccharomyces cerevisiae The study of eukaryotic membrane proteins has been hampered by a paucity of systems that achieve consistent high-level functional protein expression.

We report the use of a modified membrane protein hyperexpression system to characterize three classes of fungal membrane proteins (ABC transporters Pdr5p, CaCdr1p, CaCdr2p, CgCdr1p, CgPdh1p, CkAbc1p, and CneMdr1p, the major facilitator The EasySelect Pichia Expression Kit provides all needed components for protein production in the yeast Pichia pastoris.

The EasySelect Kit contains Pichia yeast strains, expression vectors, and reagents that enable transformation of Pichia cells. Selection with Zeocin antibiotic makes it easy to sc.

PCR cloning for Z. bailii GAS 1 homologue. Because of the high phylogenetic correlation between Z. bailii and S. cerevisiae[] and because of the high percentage of identity among the few known Z. bailii and the corresponding S. cerevisiae gene sequences, our first attempt was to clone the gene homologous to GAS 1 in Z.

bailii by PCR amplification, using primers designed on the 3' and 5' ends The Vickers group currently works in yeast (Saccharomyces cerevisiae), Escherichia coli, cyanobacteria (Synechocystis and Synechococcus spp.), Pseudomonas putida, and plants (various species).

These diverse areas are linked though application of synthetic biology to understand fundamental biology, and applying this understanding to industrial   Sustainable production of chemicals, materials, and pharmaceuticals is increasingly performed by genetically engineered cell factories.

Engineering of complex metabolic routes or cell behavior control systems requires robust and predictable gene expression tools.

In this challenging task, orthogonality is a fundamental prerequisite for such ://

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